Journal: Frontiers in Synaptic Neuroscience

Article Title: Identification of Synaptic DGKθ Interactors That Stimulate DGKθ Activity

doi: 10.3389/fnsyn.2022.855673

Figure Lengend Snippet: Validation of candidate DGKθ-interacting proteins. (A) Streptavidin beads were used to isolate biotinylated proteins from DGKθ-GFP-APEX2 labeled cortical neuron lysates (DIV21), and biotinylation was confirmed biochemically for dynamin-1, synaptogyrin-1, Munc18-1, clathrin heavy chain 1, Syt1, CaMKIIα, Hsc70, and syntaxin-7. (B) DGKθ overexpressed in HEK cells is immunoprecipitated with a myc antibody. (C) DGKθ interacts with Hsc70, CaMKIIα, Syt1, synaptogyrin-1, dynamin-1, syntaxin-7, and Munc18-1 when both are overexpressed in HEK cells. Hsc70 interacts with DGKθ at its endogenous expression level. (D) Clathrin heavy chain 1 does not interact with DGKθ when overexpressed in HEK cells. All blots are representative of at least three separate experiments.

Article Snippet: Hspa8 , Origene , TP302209 , , .

Techniques: Biomarker Discovery, Labeling, Immunoprecipitation, Expressing

Journal: Frontiers in Synaptic Neuroscience

Article Title: Identification of Synaptic DGKθ Interactors That Stimulate DGKθ Activity

doi: 10.3389/fnsyn.2022.855673

Figure Lengend Snippet:

Article Snippet: Hspa8 , Origene , TP302209 , , .

Techniques: Purification

Journal: Frontiers in Synaptic Neuroscience

Article Title: Identification of Synaptic DGKθ Interactors That Stimulate DGKθ Activity

doi: 10.3389/fnsyn.2022.855673

Figure Lengend Snippet:

Article Snippet: Hspa8 , Origene , TP302209 , , .

Techniques:

Journal: American Journal of Translational Research

Article Title: CircRNAs in exosomes from high glucose-treated glomerular endothelial cells activate mesangial cells

doi:

Figure Lengend Snippet: Isolation and identification of exosomes from glomerular endothelial cells treated with high glucose and low glucose. A: Exosomal size analysis of HG-GEC-EXO and NG-GEC-EXO by NTA. B: The number of exosomes analyzed in the two different groups by NTA. C: Western blot analysis of HspA8 and Alix in the exosomes of the two different groups. ****P-value < 0.001. HG-GEC-EXO: exosomes from glomerular endothelial cells treated with high glucose. NG-GEC-EXO: exosomes from glomerular endothelial cells treated with normal (low) glucose; NTA: Nanoparticle tracer analysis.

Article Snippet: After blocking for 1 h at room temperature, the membrane was incubated with rabbit polyclonal anti-mouse HspA8 (1:1000) and Alix (1:1500) antibody (BOSTER, USA) for 12 hours.

Techniques: Isolation, Western Blot